GPR65 (IHC-grade), G Protein-Coupled Receptor 65 Antibody

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GPR65 (IHC-grade), G Protein-Coupled Receptor 65 Antibody

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Order number: 7TM0113N-IC
Content: 100 µl
Host: Rabbit

The GPR65 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

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The GPR65 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP65. It can be used to detect total GPR65 receptors in Western blots independent of phosphorylation. The GPR65 antibody can also be used to isolate and enrich GPR65 receptors from brain lysates. It also detects GPR65 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names Dig1 | Gpcr25 | Psychosine... more

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Alternative Names	Dig1 \| Gpcr25 \| Psychosine receptor \| TDAG8 \| T cell death associated protein 8
IUPHAR Target ID	113
UniProt ID	Q8IYL9
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents distal end of carboxy-terminal tail of human GPR65.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 87 in kidney.... more

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Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 87 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR87 (G Protein-coupled Receptor 87) antibody (7TM0122N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, GPR87 receptors were detected at the plasma membrane of collecting duct cells in kidney.

Figure 2. Validation of the G protein-coupled receptor 87 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the G protein-coupled receptor 87 (GPR87) were lysed and immunoblotted with the phosphorylation-independent anti-GPR87 antibody (7TM0122N-IC) at a dilution of 1:1000.