CCR7 (IHC-grade), CC Chemokine Receptor 7 Antibody

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CCR7 (IHC-grade), CC Chemokine Receptor 7 Antibody

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Order number: 7TM0064N-IC
Content: 100 µl
Host: Rabbit

The CCR7 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The CCR7 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human CCR7 . It can be used to detectCCR7 receptors in Western blots in a phosphorylation-sensitive manner. Total CXCR4 can be detected in Western blots and immunohistochemistry only after phosphatase treatment. It can also be used to isolate and enrich CCR7 receptors from brain lysates. It also detects CCR7 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names CCR7, CC Chemokin Receptor 7 IUPHAR Target ID... more

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Alternative Names	CCR7, CC Chemokin Receptor 7
IUPHAR Target ID	64
UniProt ID	P32248 (human)
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human CCR7.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of CXC Chemokine Receptor 4 in human spleen.... more

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Figure 1. Immunohistochemical identification of CXC Chemokine Receptor 4 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CCR7 (CC Chemokine Receptor 7) antibody (7TM0064N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of CC Chemokine Receptor 7 in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CCR7 (CC Chemokine receptor 7) antibody (7TM0064N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Validation of the CC Chemokine Receptor 7 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the CC Chemokine Receptor 7 (CCR7) were lysed and immunoblotted with the anti-CCR7 antibody (7TM0064N-IC) at a dilution of 1:1000.

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