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GCGR (non-phospho) Glucagon Receptor Antibody

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  • 7TM0251N
  • 100 µl
  • Rabbit
The non-phospho GCGR antibody is directed against the distal end of the carboxyl-terminal tail... more

The non-phospho GCGR antibody is directed against the distal end of the carboxyl-terminal tail human GCGR. It can be used to detect total GCGR receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich GCGR receptors from tissue lysates. It also detects GCGR in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names GGR IUPHAR Target ID   251... more

 

Alternative Names GGR
IUPHAR Target ID  251
UniProt ID  P47871
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide corresponding to c-terminal end of human GCGR.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the Glucagon Receptor  in transfected HEK293 cells. Native... more

Figure 1. Validation of the Glucagon Receptor  in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Glucagon Receptor  (GCGR) were lysed and immunoblotted with the anti-GCGR antibody (7TM0251N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of the Glucagon Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GCGR (Glucagon Receptor) antibody (7TM0251N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. 

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