pT336/pT338-LPA4 (phospho-Lysophosphatidic Acid Receptor 4 Antibody)

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Agonist-induced Threonine336/Threonine338 phosphorylation of the Lysophosphatidic Acid Receptor 4

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Order number: 7TM0094A
Content: 100 µl
Host: Rabbit

Threonine336/Threonine338 (T336/T338) is major phosphorylation site of the Lysophosphatidic... more

Threonine336/Threonine338 (T336/T338) is major phosphorylation site of the Lysophosphatidic Acid Receptor 4 (LPA4). The pT336/pT338-LPA4 antibody detects phosphorylation in response to agonists. T336/T338 phosphorylation is likely to be involved in efficient ligand sequestration by LPA.

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Download 7TM Western Blot Protocol

Alternative Names P2RY9, P2Y5-like receptor, GPR23, Purinergic... more

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Alternative Names	P2RY9, P2Y5-like receptor, GPR23, Purinergic receptor 9
IUPHAR Target ID	94
UniProt ID	Q99677
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human LPA4 around the phosphorylation site of Thr336/Thr338.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine336/Threonine338 phosphorylation of the Lysophosphatidic... more

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Figure 1. Agonist-induced Threonine336/Threonine338 phosphorylation of the Lysophosphatidic Acid Receptor 4. Upper panel, HEK293 cells stably expressing the LPA4-Receptor (LPA4) were either not exposed or exposed to 1 µM of LPA4-Receptor agonists 1-oleoyl-LPA for 30 minutes. Cells were lysed and immunoblotted with the anti-pT336/pT338-LPA4 antibody (7TM0094A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-LPA4 antibody to confirm equal loading of the gel.

Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1... more

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Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.

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