GCGR (IHC-grade), Glucagon Receptor Antibody

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Preview: Immunohistochemical identification of the Glucagon Receptor in pancreas

Preview: Validation of the Glucagon Receptor in transfected HEK293 cells

Immunohistochemical identification of the Glucagon Receptor in pancreas

Validation of the Glucagon Receptor in transfected HEK293 cells

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Delivery time appr. 5-8 days

Order number: 7TM0251N-IC
Content: 100 µl
Host: Rabbit

The GCGR antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The GCGR antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GCGR. It can be used to detect total GCGR receptors in Western blots independent of phosphorylation. The GCGR antibody can also be used to isolate and enrich GCGRreceptors from brain lysates. It also detects GCGR in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names GGR IUPHAR Target ID 251... more

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Alternative Names	GGR
IUPHAR Target ID	251
UniProt ID	P47871
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GCGR.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of the... more

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Figure 1. Immunohistochemical identification of the Glucagon Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GCGR (Glucagon Receptor) antibody (7TM0251N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Validation of the Glucagon Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Glucagon Receptor (GCGR) were lysed and immunoblotted with the anti-GCGR antibody (7TM0251N-IC) at a dilution of 1:1000.

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