FPR2 (IHC-grade), Formylpeptide Receptor 2 Antibody

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Preview: Immunohistochemical identification of the Formylpeptide Receptor 2 in striatum

Preview: Validation of the Formylpeptide Receptor 2 in transfected HEK293 cells

Immunohistochemical identification of the Formylpeptide Receptor 2 in striatum

Validation of the Formylpeptide Receptor 2 in transfected HEK293 cells

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Order number: 7TM0223N-IC
Content: 100 µl
Host: Rabbit

The FPR2 antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The FPR2 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human FPR2. It can be used to detect total FPR2 receptors in Western blots independent of phosphorylation. The FPR2 antibody can also be used to isolate and enrich FPR2 receptors from brain lysates. It also detects FPR2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names ALXR, FPRL1, FPRH2, Formyl-peptide Receptor-like 1,... more

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Alternative Names	ALXR, FPRL1, FPRH2, Formyl-peptide Receptor-like 1, Formyl-peptide Receptor 2
IUPHAR Target ID	223
UniProt ID	P25090
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human FPR2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of the... more

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Figure 1. Immunohistochemical identification of the Formylpeptide Receptor 2 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FPR2 (Formylpeptide Receptor 2) antibody (7TM0223N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Validation of the Formylpeptide Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Formylpeptide Receptor 2 (FPR2) were lysed and immunoblotted with the anti-FPR2 antibody (7TM0223N-IC) at a dilution of 1:1000.