pT323/pS324/pS325-LPA2 (phospho-Lysophosphatidic Acid Receptor 2 Antibody)

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Agonist-induced Threonine323/Serine324/Serine325 phosphorylation of the Lysophosphatidic Acid Receptor 2

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Order number: 7TM0273A
Content: 100 µl
Host: Rabbit

Threonine323/Serine324/Serine325 (T323/S324/325) is major phosphorylation site of... more

Threonine323/Serine324/Serine325 (T323/S324/325) is major phosphorylation site of the Lysophosphatidic Acid Receptor 2 (LPA2). pT323/pS324/pS325-LPA2 antibody detects phosphorylation in response to agonists. T323/S324/325 phosphorylation is likely to be involved in efficient ligand sequestration by LPA.

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Alternative Names Edg-4, endothelial differentiation gene 4,... more

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Alternative Names	Edg-4, endothelial differentiation gene 4, PBX4, pre-B-cell leukemia transcription factor 4
IUPHAR Target ID	273
UniProt ID	Q9HBW0
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human LPA2 around the phosphorylation site of Thr323/Ser324/Ser325.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine323/Serine324/Serine325 phosphorylation of the... more

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Figure 1. Agonist-induced Threonine323/Serine324/Serine325 phosphorylation of the Lysophosphatidic Acid Receptor 2. Upper panel, HEK293 cells stably expressing the LPA2-Receptor (LPA2) were either not exposed or exposed to 1 µM of LPA2-Receptor agonists 1-oleoyl-LPA for 30 minutes. Cells were lysed and immunoblotted with the anti-pT323/pS324/pS325-LPA2 antibody (7TM0273A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-LPA2 antibody to confirm equal loading of the gel.

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