pT332/pS335-LPA6 (phospho-Lysophosphatidic Acid Receptor 6 Antibody)

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Agonist-induced Threonine332/Serine335 phosphorylation of the Lysophosphatidic Acid Receptor 6

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Order number: 7TM0163A
Content: 100 µl
Host: Rabbit

Threonine332/Serine335 (T332/S335) is major phosphorylation site of the Lysophosphatidic... more

Threonine332/Serine335 (T332/S335) is major phosphorylation site of the Lysophosphatidic Acid Receptor 6 (LPA6). pT332/pS335-LPA6 antibody detects phosphorylation in response to agonists. T332/S335 phosphorylation is likely to be involved in efficient ligand sequestration by LPA.

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Alternative Names P2RY5, oleoyl-L-alpha-lysophosphatidic acid... more

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Alternative Names	P2RY5, oleoyl-L-alpha-lysophosphatidic acid receptor, P2Y purinoceptor 5
IUPHAR Target ID	163
UniProt ID	P43657
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human LPA6 around the phosphorylation site of Thr332/Ser335.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine332/Serine335 phosphorylation of the Lysophosphatidic Acid... more

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Figure 1. Agonist-induced Threonine332/Serine335 phosphorylation of the Lysophosphatidic Acid Receptor 6. Upper panel, HEK293 cells stably expressing the LPA6-Receptor (LPA6) were either not exposed or exposed to 1 µM of LPA6-Receptor agonists 1-oleoyl-LPA for 30 minutes. Cells were lysed and immunoblotted with the anti-pT332/pS335-LPA6 antibody (7TM0163A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-LPA6 antibody to confirm equal loading of the gel.

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