GPR25 (IHC-grade), G Protein-Coupled Receptor 25 Antibody

Figure 1. Immunohistochemical identification of GPR25 Receptor in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR25 (GPR25 Receptor) antibody (7TM0095N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the GPR25 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR25 Receptor (GPR25) were lysed and immunoblotted with the phosphorylation-independent anti-GPR25 antibody (7TM0095N-IC) at a dilution of 1:1000.

Figure 3. Immunohistochemical varification of  GPR25 Receptor antibody in duodenum. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR25 (non-phospho-GPR25 Receptor)) antibody (7TM0095N-IC). Sections were then incubated with anti-GPR25 (non-phospho-GPR25 Receptor) antibody (7TM0095N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR25 receptors were uniformly detected at the plasma membrane of cells.