β2 (non-phospho), β2-Adrenoceptor Antibody

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Validation of the β2-Adrenoceptor in transfected HEK293 cells

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Delivery time appr. 5-8 days

Order number: 7TM0029N
Content: 100 µl
Host: Rabbit

The non-phospho- β2 antibody is directed against the third intracellular loop of human β2 . It... more

The non-phospho-β2 antibody is directed against the third intracellular loop of human β2. It can be used to detect total β2 receptors in Western blots independent of phosphorylation. The non-phospho-β2 antibody can also be used to isolate and enrich β2 receptors from cell and tissue lysates.

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Alternative Names β2,B2AR, ADRB2R, β2 Adrenergic... more

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Alternative Names	β2,B2AR, ADRB2R, β2 Adrenergic Receptor, β2-Adrenoceptor
IUPHAR Target ID	29
UniProt ID	P07550
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide derived from human β2 with the sequence GLRRSSKFLK.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the β2-Adrenoceptor in transfected HEK293 cells. Native... more

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Figure 1. Validation of the β2-Adrenoceptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the β2-Adrenoceptor (β2) were lysed and immunoblotted with the phosphorylation-independent anti-β2 antibody (7TM0029N-WB) at a dilution of 1:1000.

Benkel T, Zimmermann M, Zeiner J, Bravo S, Merten N, Lim VJY, Matthees ESF, Drube J,... more

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Benkel T, Zimmermann M, Zeiner J, Bravo S, Merten N, Lim VJY, Matthees ESF, Drube J, Miess-Tanneberg E, Malan D, Szpakowska M, Monteleone S, Grimes J, Koszegi Z, Lanoiselée Y, O'Brien S, Pavlaki N, Dobberstein N, Inoue A, Nikolaev V, Calebiro D, Chevigné A, Sasse P, Schulz S, Hoffmann C, Kolb P, Waldhoer M, Simon K, Gomeza J, Kostenis E. How Carvedilol activates β2-adrenoceptors. Nat Commun. 2022 Nov 19;13(1):7109. doi: 10.1038/s41467-022-34765-w. PMID: 36402762; PMCID: PMC9675828.

Burghi V, Paradis JS, Officer A, Adame-Garcia SR, Wu X, Matthees ESF, Barsi-Rhyne B, Ramms DJ, Clubb L, Acosta M, Tamayo P, Bouvier M, Inoue A, von Zastrow
M, Hoffmann C, Gutkind JS. Gαs is dispensable for β-arrestin coupling but dictates GRK selectivity and is predominant for gene expression regulation by
β2-adrenergic receptor. J Biol Chem. 2023 Nov;299(11):105293. doi:10.1016/j.jbc.2023.105293. Epub 2023 Sep 27. PMID: 37774973; PMCID: PMC10641165.

Radoux-Mergault A, Oberhauser L, Aureli S, Gervasio FL, Stoeber M.Subcellular location defines GPCR signal transduction. Sci Adv. 2023 Apr 21;9(16):eadf6059. doi: 10.1126/sciadv.adf6059. Epub 2023 Apr 19. PMID: 37075112; PMCID: PMC10115417.

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