Premium Phosphosite-Specific 7TM Antibodies
Novel Tools for Your GPCR Research
Select Your Country of Delivery below

Premium Phosphosite-Specific 7TM Antibodies

Phosphorylation of intracellular serine and threonine residues is the most important post translational modification of G protein-coupled receptors (GPCRs) also called heptahelical or seven transmembrane receptors (7TMR). After agonist exposure, these receptors acquire an active conformation, which is recognized by a family of highly specialized GPCR kinases (GRKs). Agonist-driven phosphorylation by GRKs regulates acute receptor desensitization, arrestin recruitment, internalization, post-activation signaling, long-term tolerance and drug addiction. Phosphosite-specific 7TM antibodies are designed to specifically detect agonist-activated GPCRs. In fact, recent work shows that ligand profiling using phosphosite-specific 7TM antibodies provides valuble information on ligand bias beyond that obtained with conventional ß-arrestin recruitment assays. Phosphosite-specific 7TM antibodies are novel tools for GPCR research that can be used to:

  • profile agonist properties of novel GPCR ligands
  • decipher the phosphorylation barcode of GPCRs
  • determine the spatial and temporal dynamics of receptor phosphorylation
  • identify relevant kinases and phosphatases for GPCR phosphorylation and dephosphoryation

Lifecycle3

Schematic representation of the G protein-coupled receptor phosphorylation / dephosphorylation cycle. GRK, G protein-coupled receptor kinase; PKC, protein kinase C; cPP1, catalytic subunit of protein phosphatase 1; R*, activated GPCR; CCP, clathrin-coated pit. 

Close filters
2 From 23
No results were found for the filter!
NEW
Agonist-induced Serine409/Serine410 phosphorylation of the Neurotensin Receptor 1
pS409/pS410-NTS1 (phospho-Neurotensin Receptor...
Serine409/Serine410 (S409/S410) is major phosphorylation site of the Neurotensin Receptor 1 (NTS1). The pS409/pS410-NTS1 antibody detects phosphorylation in response to agonists. S409/S410 phosphorylation is likely to be involved in...
$ 400.00 *
Details
NEW
pT329/pT330-BLT2 (phospho-Leukotriene Receptor BLT2 Antibody)
pT329/pT330-BLT2 (phospho-Leukotriene Receptor...
Threonine329/Threonine330 (T329/T330) is a major phosphorylation site of the BLT2 receptor. The pT329/pT330-BLT2 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. T329/T330...
$ 400.00 *
Details
NEW
Agonist-induced Serine320/Threonine324 phosphorylation of the Leukotriene Receptor BLT2.
pS320/pT324-BLT2 (phospho-Leukotriene Receptor...
Serine320/Threonine324 (S320/T324) is a major phosphorylation site of the BLT2 receptor. The pS320/pT324-BLT2 antibody detects phosphorylation in response to high- and low-efficacy agonists and after PKC activation. S320/T324...
$ 400.00 *
Details
NEW
Agonist-induced Serine377/Ser378 phosphorylation of the Oxytocin Receptor.
pS377/pS378-OT (phospho-Oxytocin Receptor...
Serine377/Serine378 (S377/S378) is major phosphorylation site of the Oxytocin Receptor (OT). The p3S77/pS378-OT antibody detects phosphorylation in response to agonists. S377/S378 phosphorylation is likely to be involved in efficient...
$ 400.00 *
Details
NEW
Agonist-induced Serine366/Serine368 phosphorylation of the Oxytocin Receptor
pS366/pS368-OT (phospho-Oxytocin Receptor...
Serine366/Serine368 (S366/S368) is major phosphorylation site of the Oxytocin Receptor (OT). The pS366/pS368-OT antibody detects phosphorylation in response to agonists. S366/S368 phosphorylation is likely to be involved in efficient...
$ 400.00 *
Details
NEW
Agonist-induced Serine345 phosphorylation of the Sphingosine 1-Phosphate Receptor 5
pS345-S1P5 (phospho-Sphingosine 1-Phosphate...
Serine345 (S345) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 5 (S1P5). The pS345-S1P5 antibody detects phosphorylation in response to agonists. S345 phosphorylation is likely to be involved in efficient ligand...
$ 400.00 *
Details
NEW
Agonist-induced Serine359/Serine361/Serine363 phosphorylation of the Sphingosine 1-Phosphate Receptor 5
pS359/pS361/pS363-S1P5 (phospho-Sphingosine...
Serine359/Serine361/Serine363 (S359/S361/S363) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 5 (S1P5). The pS359/pS361/pS363-S1P5 antibody detects phosphorylation in response to agonists. S359/S361/S363...
$ 400.00 *
Details
NEW
pS364/pS366/pS368-S1P4 (phospho-Sphingosine 1-Phosphate Receptor 4 Antibody)
pS364/pS366/pS368-S1P4 (phospho-Sphingosine...
Serine364/Serine366/Serine368 (S364/S366/S368) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 4 (S1P4). The pS364/pS366/pS368-S1P4 antibody detects phosphorylation in response to agonists. S364/S366/S368...
$ 400.00 *
Details
NEW
pT442/pS443-GIPR (phospho-Gastric Inhibitory Polypeptide Receptor Antibody)
pT442/pS443-GIPR (phospho-Gastric Inhibitory...
Threonine442/Serine443 (T442/S443) is major phosphorylation site of human GIP receptor. The pT442/pS443-GIPR antibody detects phosphorylation in response to agonists. T442/S443 phosphorylation is likely to be involved in efficient ligand...
$ 400.00 *
Details
NEW
Agonist-induced Threonine358/Serine360 phosphorylation of GPR132
pT358/pS360-GPR132 (phospho-GPR132 Antibody)
Threonine358/Serine360 (T358/S360) is major phosphorylation site of human GPR132 receptor. The pT358/pS360-GPR132 antibody detects phosphorylation in response to agonists. T358/S360 phosphorylation is likely to be involved in efficient...
$ 400.00 *
Details
NEW
Agonist-induced Threonine344/Serine349 phosphorylation of GPR132
pT344/pS349-GPR132 (phospho-GPR132 Antibody)
Threonine344/Serine349 (T344/S349) is major phosphorylation site of human GPR132 receptor. The pT344/pS349-GPR132 antibody detects phosphorylation in response to agonists. T344/S349 phosphorylation is likely to be involved in efficient...
$ 400.00 *
Details
NEW
Agonist-induced Threoine339/Serine341 phosphorylation of GPR132
pT339/pS341-GPR132 (phospho-GPR132 Antibody)
Threonine339/Serine341 (T339/S341) is major phosphorylation site of human GPR132 receptor. The pT339/pS341-GPR132 antibody detects phosphorylation in response to agonists. T339/S341 phosphorylation is likely to be involved in efficient...
$ 400.00 *
Details
NEW
Agonist-induced Serine335/Serine337 phosphorylation of the Sphingosine 1-Phosphate Receptor 3
pS335/pS337-S1P3 (phospho-Sphingosine...
Serine335/Serine337 (S335/S337) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 3 (S1P3). The pS335/pS337-S1P3 antibody detects phosphorylation in response to agonists. S335/S337 phosphorylation is likely to be...
$ 400.00 *
Details
NEW
pS335/pS336-CB2 (phospho-Cannabinoid Receptor 2 Antibody)
pS335/pS336-CB2 (phospho-Cannabinoid Receptor 2...
Serine335/Serine336 (S335/S336) is major phosphorylation site of the Cannabinoid Receptor 2 (CB2). The pS335/pS336-CB2 antibody detects phosphorylation in response to agonists. S335/S336 phosphorylation is likely to be involved in...
$ 400.00 *
Details
NEW
gonist-induced Threonine357/Serine360 phosphorylation of the Neuropeptide Y receptor 1
pT357/pS360-NPY1 (phospho-Neuropeptide Y...
Threonine357/Serine360 (T357/S360) is a major phosphorylation site of the Neuropeptide Y Receptor 1. The pT357/pS360-NPY1 antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation....
$ 400.00 *
Details
NEW
Agonist-induced Serine594/Threonine597/Serine599 phosphorylation of the SMO Receptor
pS594/pT597/pS599-SMO (phospho-SMO Receptor...
Serine594/Threonine597/Serine599 (S594/S597/S599) is major GRK2 phosphorylation site of the SMO receptor. The pS594/pT597/pS599-SMO antibody detects phosphorylation in response to agonists. The pS594/pT597/pS599-SMO antibody can be used...
$ 400.00 *
Details
2 From 23

For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

For further reading refer to: Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636.... read more »
Close window
Premium Phosphosite-Specific 7TM Antibodies

For further reading refer to:

Kliewer A, Reinscheid RK, Schulz S. Emerging Paradigms of G Protein-Coupled Receptor Dephosphorylation. Trends Pharmacol Sci. 2017 Jul;38(7):621-636. doi:10.1016/j.tips.2017.04.002. Epub 2017 May 4. Review. PubMed PMID: 28478994.

Miess E, Gondin AB, Yousuf A, Steinborn R, Mösslein N, Yang Y, Göldner M, Ruland JG, Bünemann M, Krasel C, Christie MJ, Halls ML, Schulz S, Canals M. Multisite phosphorylation is required for sustained interaction with GRKs and arrestins during rapid μ-opioid receptor desensitization. Sci Signal. 2018 Jul 17;11(539). pii: eaas9609. doi: 10.1126/scisignal.aas9609. PubMed PMID: 30018083.

Kliewer A, Schmiedel F, Sianati S, Bailey A, Bateman JT, Levitt ES, Williams JT, Christie MJ, Schulz S. Phosphorylation-deficient G-protein-biased μ-opioid receptors improve analgesia and diminish tolerance but worsen opioid side effects. Nat Commun. 2019 Jan 21;10(1):367. doi: 10.1038/s41467-018-08162-1. PubMed PMID: 30664663; PubMed Central PMCID: PMC6341117.

Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

Glück L, Loktev A, Moulédous L, Mollereau C, Law PY, Schulz S. Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5. Biol Psychiatry. 2014 Nov 15;76(10):767-74. doi: 10.1016/j.biopsych.2014.01.021. Epub 2014 Feb 3. PubMed PMID: 24629717; PubMed Central PMCID: PMC4119866.

Recently viewed