CB2 (non-phospho) Cannabinoid Receptor 2 Antibody

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Preview: Validation of the Cannabinoid Receptor 2 in transfected HEK293 cells

Preview: Immunohistochemical identification of the Cannabinoid Receptor 2 in kidney

Validation of the Cannabinoid Receptor 2 in transfected HEK293 cells

Immunohistochemical identification of the Cannabinoid Receptor 2 in kidney

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Order number: 7TM0057N
Content: 100 µl
Host: Rabbit

The non-phospho-CB2 receptor antibody is directed against the distal end of the... more

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The non-phospho-CB2 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human CB2. It can be used to detect total CB2 receptors in Western blots independent of phosphorylation. The CB2 antibody can also be used to isolate and enrich CB2 receptors from tissue lysates. It also detects CB2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names CB2-R, Peripheral Cannabinoid Receptor IUPHAR... more

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Alternative Names	CB2-R, Peripheral Cannabinoid Receptor
IUPHAR Target ID	57
UniProt ID	P34927
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human CB2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Cannabinoid Receptor 2... more

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Figure 1. Validation of the Cannabinoid Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Cannabinoid Receptor 2 (CB2) were lysed and immunoblotted with the anti-CB2 antibody (7TM0057N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of the Cannabinoid Receptor 2 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CB2 (Cannabinoid Receptor 2) antibody (7TM0057N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Fritzwanker S, Nagel F, Kliewer A, Stammer V, Schulz S. In situ visualization of opioid and... more

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Fritzwanker S, Nagel F, Kliewer A, Stammer V, Schulz S. In situ visualization of opioid and cannabinoid drug effects using phosphosite-specific GPCR antibodies. Commun Biol. 2023 Apr 15;6(1):419. doi: 10.1038/s42003-023-04786-2. PMID: 37061609; PMCID: PMC10105690.

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