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- Order number: 7TM0118N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR82 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR82. It can be used to detect total GPR82 receptors in Western blots independent of phosphorylation. The GPR82 antibody can also be used to isolate and enrich GPR82 receptors from tissue lysates. It also detects GPR82 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | |
| IUPHAR Target ID | 118 |
| Uniprot ID | Q96P67 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR82. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the GPR82 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR82 Receptor (GPR82) were lysed and immunoblotted with the phosphorylation-independent anti-GPR82 antibody (7TM0118N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of G Protein-coupled Receptor 82 in hippocampus. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR82 (G Protein-coupled Receptor 82) antibody (7TM0118N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 3. Immunohistochemical varification of GPR82 Receptor antibody in hippocampus. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR82 (non-phospho-GPR82 Receptor)) antibody (7TM0118N). Sections were then incubated with anti-GPR82 (non-phospho-GPR82 Receptor) antibody (7TM0118N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR82 receptors were uniformly detected at the plasma membrane of cells.
Figure 4. Immunohistochemical varification of GPR82 Receptor antibody in heart. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR82 (non-phospho-GPR82 Receptor)) antibody (7TM0118N). Sections were then incubated with anti-GPR82 (non-phospho-GPR82 Receptor) antibody (7TM0118N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR82 receptors were uniformly detected at the plasma membrane of cells.