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- Order number: 7TM0054N
- Content: 100 µl
- Host: Rabbit
The non-phospho-CaS Calcium-sensing Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human CaS. It can be used to detect total CaS receptors in Western blots independent of phosphorylation. The non-phospho-CaS antibody can also be used to isolate and enrich CaS receptors from cell and tissue lysates. It also detects CaS in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | CaR, GPRC2A, extracellular calcium-sensing receptor, divalent cation-sensing receptor |
| IUPHAR Target ID | 54 |
| UniProt ID | P41180 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents carboxyl-terminal tail of human CaS. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the Calcium-sensing Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Calcium-sensing receptor (CaS) were lysed and immunoblotted with the phosphorylation-independent anti-CaS antibody (7TM0054N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of Calcium-sensing Receptor in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CaS (Calcium-sensing receptor) antibody (7TM0054N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.
Figure 3. Immunohistochemical identification of Calcium-sensing Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CaS (Calcium-sensing receptor) antibody (7TM0054N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.