D5 (IHC-grade), Dopamine Receptor 5 Antibody

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Preview: Immunohistochemical identification of Dopamine Receptor 5 in human cortex

Preview: Immunohistochemical identification of Dopamine Receptor 5 in mouse hippocampus

Preview: Validation of the Dopamine Receptor 5 in transfected HEK293 cells

Immunohistochemical identification of Dopamine Receptor 5 in human cortex

Immunohistochemical identification of Dopamine Receptor 5 in mouse hippocampus

Validation of the Dopamine Receptor 5 in transfected HEK293 cells

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Order number: 7TM0218N-IC
Content: 100 µl
Host: Rabbit

The D5 receptor antibody is directed against the distal part of the carboxyl-terminal tail of... more

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The D5 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human D5. It can be used to detect total D5 receptors in Western blots independent of phosphorylation. The D5 antibody can also be used to isolate and enrich D5 receptors from cell and tissue lysates. It also detects D5 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names D5, DRD1B, Dopamine Receptor 5, D(1B)Dopamine Receptor... more

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Alternative Names	D5, DRD1B, Dopamine Receptor 5, D(1B)Dopamine Receptor
IUPHAR Target ID	218
UniProt ID	P21918
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human,Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human D1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Dopamine Receptor 5 in human cortex.... more

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Figure 1. Immunohistochemical identification of Dopamine Receptor 5 in human cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D5 (Dopamine Receptor 5) antibody (7TM0218N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of Dopamine Receptor 5 in mouse hippocampus. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D5 (Dopamine Receptor 5) antibody (7TM0218N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Validation of the Dopamine Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the dopamine receptor 5 (D5) were lysed and immunoblotted with the phosphorylation-independent anti-D5 antibody (7TM0218N-IC) at a dilution of 1:1000.

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