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- Order number: 7TM0220N
- Content: 100 µl
- Host: Rabbit
The non-phospho-ETB receptor antibody is directed against the distal part of the carboxyl-terminal tail of human Endothelin Receptor B (ETB). It can be used to detect total ETB receptors in Western blots independent of phosphorylation. The non-phospho-ETB antibody can also be used to isolate and enrich ETB receptors from cell and tissue lysates.
Alternative Names | HSCR, ET-RB, EDNRB |
IUPHAR Target ID | 220 |
UniProt ID | P24530(human), P48302 (mouse) |
Western Blot (WB) | 1:1000 |
Immunohistochemistry (IHC) | - |
Species Reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic peptide presents the carboxyl-terminal tail of human ETB. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Indentification of the Endothelin Receptor B in mouse tissues. Native mouse tissues were lysed and GPCR receptors were enriched by using WGA-beads (see 7TM Western Blot Protocol). Extracted proteins were immunoblotted with the phosphorylation-independent anti-ETB antibody (7TM00220N) at a dilution of 1:1000.
Figure 2. Validation of the Endothelin Receptor B in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Endothelin Receptor B (ETB) were lysed and immunoblotted with the phosphorylation-independent anti-ETB antibody (7TM00220N) at a dilution of 1:1000.