pT377-GPR88 (phospho-GPR88 Antibody)

NEW

Agonist-induced Threonine377 phosphorylation of GPR88-Receptor

$ 400.00 *

Ready to ship today,
Delivery time appr. 5-8 days

Order number: 7TM0123D
Content: 100 µl
Host: Rabbit

Threonine377 (T377) is a major phosphorylation site of the human GPR88 receptor.... more

Threonine377 (T377) is a major phosphorylation site of the human GPR88 receptor. The pT377-GPR88 antibody detects phosphorylation in response to high-efficacy agonists. T377 phosphorylation is a key regulator of human GPR88 desensitization, β-arrestin recruitment and internalization.

Available downloads:

Alternative Names STRG, striatum-specific G-protein coupled receptor... more

Close menu

Alternative Names	STRG, striatum-specific G-protein coupled receptor
IUPHAR Target ID	123
UniProt ID	Q9GZN0
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR88 around the phosphorylation site Thr377.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine377 phosphorylation of GPR88-Receptor. Upper panel,... more

Close menu

Figure 1. Agonist-induced Threonine377 phosphorylation of GPR88-Receptor. Upper panel, HEK293 cells stably expressing the GPR88 receptor (GPR88) and transiently GRK2 (Ratio GPR88 receptor:GRK2 = 10:1) were preincubated with serum-free media. After 2 hours cells were either not exposed or exposed to 100 nM of 2-PCCA for 30 minutes. Cells were lysed and immunoblotted with the anti-pT377-GPR88 antibody (7TM0123D) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-GPR88 antibody (7TM0123N) to confirm equal loading of the gel

Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA,... more

Close menu

Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA, Milligan G. Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor. J Biol Chem. 2022 Mar;298(3):101655. doi: 10.1016/j.jbc.2022.101655. Epub 2022 Jan 29. PMID: 35101446; PMCID: PMC8892012.

Ganguly A, Quon T, Jenkins L, Joseph B, Al-Awar R, Chevigne A, Tobin AB, Uehling DE, Hoffmann C, Drube J, Milligan G. G protein-receptor kinases 5/6 are the key regulators of G protein-coupled receptor 35-arrestin interactions. J Biol Chem. 2023 Oct;299(10):105218. doi: 10.1016/j.jbc.2023.105218. Epub 2023 Sep 1. PMID: 37660910; PMCID: PMC10520886.

Close menu

Read, write and discuss reviews... more

Close menu

Customer evaluation for "pT377-GPR88 (phospho-GPR88 Antibody)"

Write an evaluation

Evaluations will be activated after verification.