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P2Y4 (IHC-grade), P2Y Purinoceptor 4 Antibody

7TM Antibodies
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Preview: Immunohistochemical identification of P2Y Purinoceptor 4 in pancreas Preview: Validation of the P2Y Purinoceptor 4 in transfected HEK293 cells
Immunohistochemical identification of P2Y Purinoceptor 4 in pancreas
Validation of the P2Y Purinoceptor 4 in transfected HEK293 cells
   
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  • Order number: 7TM0325N-IC
  • Content: 100 µl
  • Host: Rabbit
Description Specifications Figure Legends Citations Downloads 4 Evaluations 0
Description
The non-phospho-P2Y4 Receptor Antibody is directed against the distal end of the... more
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The non-phospho-P2Y4 Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human P2Y4. It can be used to detect total P2Y4 receptors in Western blots independent of phosphorylation. The non-phospho-P2Y4 antibody can also be used to isolate and enrich P2Y4 receptors from cell and tissue lysates. It also detects P2Y4 in cultured cells and tissue sections by immunohistochemistry.

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Specifications
  Alternative Names P2Y4, P2Y Purinoceptor 4, Pyrimidinergic Receptor P2Y4... more
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Alternative Names P2Y4, P2Y Purinoceptor 4, Pyrimidinergic Receptor P2Y4
IUPHAR Target ID  325
UniProt ID  P51582
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents carboxyl-terminal tail of human P2Y4.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure Legends
Figure 1. Immunohistochemical identification of P2Y Purinoceptor 4 in pancreas.... more
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Figure 1. Immunohistochemical identification of P2Y Purinoceptor 4 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-P2Y4 (P2Y Purinoceptor 4) antibody (7TM0325N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the P2Y Purinoceptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the complement P2Y Purinoceptor 1 (P2Y4) were lysed and immunoblotted with the phosphorylation-independent anti-P2Y4 antibody (7TM0325N-IC) at a dilution of 1:1000

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