SST4 [UMB9] (RM-IHC-grade), Somatostatin Receptor 4 Antibody, Rabbit Monoclonal

Figure 1. Immunohistochemical identification of Somatostatin Receptor 4 in parathyroid adenoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST4 (non-phospho-Somatostatin Receptor 4) antibody (7TM0358N-ICRM) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST4 receptors were detected at the plasma membrane of nearly all cells in human parathyroid adenoma.

Figure 2. Validation of the Somatostatin Receptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 4 (SST4) were lysed and immunoblotted with the phosphorylation-independent anti-SST4 antibody (7TM0358N-ICRM) at a dilution of 1:1000.

Figure 3. Immunohistochemical varification of Somatostatin Receptor 4 antibody in human cortex. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-SST4 (non-phospho-Somatostatin Receptor 4) antibody (7TM0358N-ICRM). Sections were then incubated with anti-SST4 (non-phospho-Somatostatin Receptor 4) antibody (7TM0258N-ICRM) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation SST4 receptors were uniformly detected at the plasma membrane of nearly all cells.